Ant

April 1, 2025

Sample information
Picture
Location
Collection date 09/18/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Small, black, ant-looking, has 6 legs and three segments(head, abdomen, head), most likely has antennas but is a bit crushed. The bug has no wings. Caught in the SPA courtyard during fall, it was about 70 degrees F. The courtyard is grassy and open.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium forceps

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

My results were in lane 5.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My testing for Wolbachia worked from start to end and had many different controls that worked. From this, I know that the process worked and the results are fine. However, during my DNA sequence analysis, the Q values were very low, so my DNA sequence may not be correct.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
NNNNNNTAAGTATAATTATTCGACTAGAATTAGGATCATGTATTTCTTTAATTAATAATGATCAAATTTATAATATCCTA GTAACAAGCCNTGCTTTTATTTTAATTTTTTTTATACTAATGCTTTTTATAATCGGGGGATTTGGAAATTTTTTATTTCC TTTAATACTAGGAACTCCAGATATACCTTACCCCAAAAAAAAAAAAAAAAAATTTGACCTCCCCCCCCATCTATTAAAAT TATTTTAGTTAAAAAATTTTATAATTCCGGGGTTGGGATGGGGNAACTATCCACCCTCCCCTACTACCTATTTTTCCTCA GGGACCATCAATTACCCTTCAAATTTTTTCCCCTNTTTTGCTGAAAATTTTTTCTTTTTAAGAGACAAAAATTTTTTTTT TTCCCTCCTCAATTAACCCCATTTAAAACTAAACCTACGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tetramorium forceps was found to be negative for Wolbachia.
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