Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | North Penn-Mansfield JSHS |
| Observations | Location – Moist dirt in thick brush Notes – Dirt was cool to the touch |
| Putative identification | Arthropoda Malacostraca Isopoda Philosciidae Philoscia Philoscia muscorum |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The negative arthropod control lane returned two defined lines, and the positive DNA control returned only one. The test lane returned just one defined line in the 1000-500bp, indicating a negative result, however because of the failed controls that result is unreliable. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Philoscia muscorum was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica