2 ANT

December 5, 2024

Sample information
Picture
Photos by: Alma R for "Ant Specimens and Wanticha for "ANT Sample Results."
Location
Collection date 11/16/2024
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations
  • Ants were very easy to squish.
  • A light red/orange yellow color.
  • Smaller than red ants.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit tweezers
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain Other
Gel images
Protocol notes

Gel lanes (Bottom gel)

  1. Sample 1 CO1
  2. Sample 2 CO1
  3. Sample 1 16S
  4. Sample 2 16S

 

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I’m confident because I extracted the DNA carefully and it shows up on my DNA ladder. It’s a medium confidence level because I think it needs to be checked out from other professions to finalize the Wolbachia positivity.
Wolbachia 16S sequence Download AB1
#4 is between 8 and 9 = 500 to 400 bp. Is also lighter than the rest of the bands. #5 is between 8 and 9 = 500 to 400 bp #6 is clear (control.)
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
#2 is on 6 = 700 bp. Seems to be the clearest. #3 is on 6 = 700 bp.
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be postive for Wolbachia.
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