Sample information
Picture	Photos by: Uilani A
Location
Collection date	11/13/2024
Captive / Cultivated?	Wild-caught
Group	Hawaii Community College
Observations	One seemed dead when I caught it, and the other was alive near Kaumana caves.
Putative identification	Arthropoda Hexapoda Insecta Diptera Culicidae
Methods
Extraction kit	none
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	I am not confident.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Culicidae was found to be negative for Wolbachia.