Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/03/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Bordenstein Lab |
| Observations | found on stem of vine in a patch of mulch under a birch tree; found in front yard on west side of house; 5:25pm; 70 degrees F; they are aphids |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman