Aphids

March 29, 2021

Sample information
Picture
Photos by: Isabella R.
Location
Collection date 10/03/2020
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

found on stem of vine in a patch of mulch under a birch tree; found in front yard on west side of house; 5:25pm; 70 degrees F; they are aphids
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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