Unknown Ant

March 29, 2021

Sample information
Picture
Photos by: Isabella R.
Location
Collection date 10/03/2020
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

1/2cm, ant, reddish brown color

found on the bark of a birch tree in a suburban front yard on the west side of house; 5:20pm
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

did not have ladder

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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