Sample information |
|
Picture |
|
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Location | |
Collection date | 10/03/2020 |
Captive / Cultivated? | Wild-caught |
Group | Bordenstein Lab |
Observations | some sort of spider sitting in its web which hangs from birch branch, southfacing side of the tree
small (~1/2cm), pointed abdomen is brown, dark thorax, pale legs |
Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | Yes |
Confidence level | Medium |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Araneae was found to be postive for Wolbachia. |