Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/03/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Bordenstein Lab |
| Observations | some sort of spider sitting in its web which hangs from birch branch, southfacing side of the tree
small (~1/2cm), pointed abdomen is brown, dark thorax, pale legs |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Araneae was found to be postive for Wolbachia. |
Water Boatman