Strawberry Shortcake

January 31, 2025

Sample information
Picture
Photos by: Ernesto V
Location
Collection date 09/26/2024
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

Green, small, and round. Tan legs.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Carabidae Harpalus Harpalus rufipes

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Wolbachia experiment lab 1 to 2 to 3 to 4 followed as stated.

1% agarose gel was 0.4g of agarose in 40 mL of buffer.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls resulted as expected, therefore confidence is high.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Harpalus rufipes was found to be negative for Wolbachia.
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