Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/19/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | Pill bug Hard exterior Lots of legs
|
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Taq Polymerase used: NEB OneTaq Hot Start PCR Done on 4/4/25 Annealing temp: 49 degrees celsius DNA Ladder used: NEB 1kb Plus DNA Ladder |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level |
Low confidence, samples did not yield any data after gel electrophoresis. Lane 1: DNA Ladder Lane 2: KM1, no bands present Lane 3: KM2, no bands present Lane 4:(+) Arthropod Control, bands present at 438bp and 708bp Lane 5: (-) Arthropod Control, bands present at 708bp Lane 6: (+) DNA Control, bands present at 438bp and 708bp Lane 7: (-) DNA Control (Water), no bands present Lane 8: DNA Ladder |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Baetis_C2.2
Formica_C11
Philaenus sp._C8
Ichneumonidae_A12
Formica_A11