Common Orb Weaver

February 19, 2025

Sample information
Picture
Photos by: Daniel R.
Location
Collection date 02/05/2025
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

Multi-colored, black white brown, and also it is a little bit hairy.
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

We used the NEB extraction kit to safely extract the DNA from our samples and our controls, and that we incubated for 1 hour instead of the recommending 2-3 hours. Used the Taw Polymerse used, and annealing temperatures for each PCR reaction. Tax polymerase used was the NEB one Taq Hot Start Quick load 2x MM u/stand buffer, annealing temperature is 55 C.

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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