Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | Multi-colored, black white brown, and also it is a little bit hairy. |
| Putative identification | Arthropoda Chelicerata Arachnida |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | We used the NEB extraction kit to safely extract the DNA from our samples and our controls, and that we incubated for 1 hour instead of the recommending 2-3 hours. Used the Taw Polymerse used, and annealing temperatures for each PCR reaction |
Results |
|
| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Protocol 1
Arthropod 2
Arthropod 1 nosy pill woodloosen
Arthropod 1 (Millipede)
Arthropod 2 – Roach