Sample information |
|
Picture |
![]() |
---|---|
Location | |
Collection date | 02/18/2025 |
Captive / Cultivated? | Captive / Cultivated |
Group | Berkshire Community College |
Observations | |
Putative identification | Arthropoda |
Methods |
|
Extraction kit | Monarch DNA extraction (NEB) |
DNA extraction location | Rear half |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | |
Buffer | |
DNA stain | |
Gel images |
|
Protocol notes | For DNA the incubation time (1 minute) could have been surpassed or underestimated. No timer was set, we based our time off of how long other students let their DNA sit for. Other than that we followed the directions/protocol the whole way through. Taq polymerase used were the New England Biolabs OneTaq Hot Start. For the PCR with the arthropod Primers the annealing temperature was 49 C. |
Results |
|
Wolbachia presence | Unknown |
Confidence level | Medium |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary |