Week 2/18 lab – Arthropod Collection

February 19, 2025

Sample information
Picture
Photos by: Elliot T.
Location
Collection date 02/18/2025
Captive / Cultivated? Captive / Cultivated
Group Berkshire Community College
Observations
Putative identification Arthropoda

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

For DNA the incubation time (1 minute) could have been surpassed or underestimated. No timer was set, we based our time off of how long other students let their DNA sit for. Other than that we followed the directions/protocol the whole way through.

Taq polymerase used were the New England Biolabs OneTaq Hot Start. For the PCR with the arthropod Primers the annealing temperature was 49 C.

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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