House Cricket

February 19, 2025

Sample information
Picture
Photos by: Rylynn W.
Location
Collection date 04/06/2025
Captive / Cultivated? Captive / Cultivated
Group Berkshire Community College
Observations
Putative identification Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Taq Polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w/ Standard Buffer (product #M0488S)

DNA Ladder used: New England BioLabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)

PCR date 4/16/25- Annealing temp 49C

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The bands in the gel gave me some confidence because they looked neatly and well done. All of the rules of the experiment were also followed while being careful with techniques.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
AGTAGGTACCTCTTTAAGTATCTTAATTCGAACGGAACTAGGACAACCAGGTTATTTAATTGGAGATGATCAAACATATA ATGTTATCGTAACTGCACATGCATTTGTCATAATTTTTTTCATGGTTATACCAATTATAATTGGTGGATTCGGAAATTGA TTAGTACCCCTAATATTAGGTGCACCCGATATAGCCTTTCCTCGAATAAACAATATAAGATTTTGACTTTTACCACCCTC ACTAACCCTTTTATTAACCAGAAGAATAGTCGAAAATGGTGCAGGGACAGGATGAACAGTTTATCCACCTTTATCAACAG GAATCGCCCACGCCGGAGCATCTGTTGATTTAGCCATTTTTTCATTACACTTAGCTGGAATTTCATCAATTCTGGGAGCC GTTAATTTCATTACAACTATAATCAATATACGAGCACCTGGGATGTCATTAGATCAAACCCCATTATTTGTATGAGCTGT TGGAATCACTGCCCTCCTCCTATTATTATCTTTACCTGTTCTTGCGGGTGCAATCACAATATTACTAACAGATCGAAATC TGAATACATCATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Acheta domesticus was found to be postive for Wolbachia.
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