Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/18/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Berkshire Community College |
| Observations | The crickets lived in a box in Petco altogether. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | For DNA extraction we could have incubated for longer than we did. Incubation time was 1 minute and we didn’t set an exact timer. Other than that, the protocol was followed. The Taq polymerase used were the New England Biolabs One Taq Hot Start. For PCR with the arthropod primers the annealing temp was 49C. Updated: For the duplex PCR the annealing temp was 55C. The Taq polymerase used-NEB OneTaq Hot Start Quick-Load 2x MM w/stand. Date we set up each PCR reaction is 3/26/25. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Black Blow Fly
arthropod 2
Rachel S. Fruit Fly
Mealworm Beetle
T. Viridis (Long-Jawed Orbweaver)