Sample information |
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Picture |
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Location | |
Collection date | 02/09/2025 |
Captive / Cultivated? | Wild-caught |
Group | Berkshire Community College |
Observations | Found in the soil in a potted plant. Body has a dark brown shade, It measures around 8 Mm and has 12 pairs of legs. |
Putative identification | Arthropoda Myriapoda Chilopoda Lithobiomorpha Henicopidae Lamyctes Lamyctes emarginatus |
Methods |
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Extraction kit | Monarch DNA extraction (NEB) |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Edvotek Gel Electrophoresis |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes | The Taq Polymerase used was the New England Biolabs OneTaq Hot Start Quick-Load 2x MM w/ Standard Buffer (Product # M0488S). The DNA Ladder used was the New England Biolabs 1 kb DNA Ladder for Safe Stains(Product # N0559S). The date the PCR for the Wolbachia Primer was set up was 3/27/2025 at 55°C. The Date for the Arthropod Primer was set up on 3/6/2025 at 49°C. Gel Result Key: 1)DNA Ladder 2)Lamyctes emarginatus 3)Genus harmonia 4)Positive Arthropod Control 5)Negative Arthropod Control 6)Positive DNA Control 7)Water Control 8)DNA Ladder |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | For the Wolbachia gel, the DNA and Water Controls are accurate except the positive Arthropod control bar is faint. Nothing else showed on the gel showing a negative result for Wolbachia. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Lamyctes emarginatus was found to be negative for Wolbachia. |