Sample information

Captive Dubia Roach sourced from dubiaroaches.com (Wichita, Kansas). Kept in enclosure and fed dry dog food and vegetables.

Methods

Taq polymerase used: New England Biolabs OneTaq HotStart Quick-Load 2x MM w/ Standard Buffer(product #M0488S)

DNA Ladder used: New England. Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)

PCR cocktail was set up on 3/27/25 using both Arthropod and Wolbachia primers at an annealing temperature of 49* Celsius.

Lanes:

1. DNA Ladder

2. ELB1-B. dubia

3. JBD1- B. dubia

4. AC+ Wolbachia positive fruit fly

5. AC – Wolbachia negative fruit fly

6. DNA Control

7. Water

Also included is a second gel set up for a single reaction with only Arthropod Primers on 3/13/25. Lanes are set up the same as the duplex reaction. ELB1 and JBD1 both had significant smearing due to too much DNA.

There is low confidence because there was still smearing in the samples. But there is still some confidence since there is faint banding at approx. 708 base pairs and the fact that the controls were successful.