Field Cricket

September 11, 2023

Sample information
Picture
Photos by: Abram k.
Location
Collection date
Captive / Cultivated?
Group Venture High School
Observations

cold, windy, rocky, found in the morning
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

we followed the recommended conditions for Wolbachia primers only. we used the standard reagents from MiniPCR.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

it matched the positive controls
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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