Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/02/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | William Fremd High School |
| Observations | This carpenter ant was found in the garage, and it was quick moving, yet easy to notice on the floor as it did not blend well with its surroundings when I located it. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | This is lane #146 in the given picture. There are multiple bands below the main band for the CO1 gene, so the gene may have been truncated, or there may have been nuclear genome repeats. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The controls worked. A band was clearly visible in the proper region. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus