NV Latrine Fly

September 11, 2023

Sample information
Picture
Location
Collection date 10/03/2021
Captive / Cultivated? Captive / Cultivated
Group Neuqua Valley High School
Observations
  • Flew in circles
  • Casually stopped at times
Putative identification Arthropoda Hexapoda Insecta Diptera Muscidae

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There is a band there, but it’s extremely faint, so we think it’s negative
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Muscidae was found to be negative for Wolbachia.
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