Sample information |
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Picture |
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Location | |
Collection date | 01/02/2025 |
Captive / Cultivated? | Wild-caught |
Group | Penn State Lehigh Valley |
Observations | In a garage |
Putative identification | Arthropoda Hexapoda Insecta Blattodea |
Methods |
|
Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Partial abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes | |
Results |
|
Wolbachia presence | Yes |
Confidence level | High |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Blattodea was found to be postive for Wolbachia. |