Woodlouse Spider

April 2, 2021

Sample information
Picture
Photos by: Dr. Edwards
Location
Collection date 03/23/2021
Captive / Cultivated? Wild-caught
Group Venture High School
Observations

This specimen was quite small for it’s species, ( about half an inch long ) leading me to believe that it was quite young.
Putative identification Arthropoda Chelicerata Arachnida Araneae Dysderidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed recommended procedure for wolbachia only primers

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident that this specimen is wolbachia negative because all my controls showed up as they should.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dysderidae was found to be negative for Wolbachia.
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