Trichoptera Sarah C. 11 March

Sample information

Picture
Photos by: Sarah C.
Location
Collection date 03/11/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Collected during late winter and was secured underneath a rock next to the stream. The temperature was 53 degrees F in an aquatic habitat. The arthropod was collected using a dip net and kicking rocks by the stream. The arthropod has a head and first three thoraxes are hardened, 2 fleshy, hairy hooks on the posterior, 3 pairs of segmented legs, gill tufts on abdomen. The specific name of the organism is Hydropsyche betteni. It’s common name is a Common Net Spinning Caddisfly. The organism was stored in ethanol after collection in a microtube.

Putative identification Arthropoda Hexapoda Insecta Trichoptera

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
Report Inappropriate Post