Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/23/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Venture High School |
| Observations | I had went to antelope island and the weather was a lil windy and cold I had found my bug on a rock |
| Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Larva |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | we followed the recommended conditions for wolbachia primers only we used the standard reagent from miniPCR |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | it matched all the positives but it looked a little off to me but it still looked really good |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Chilopoda was found to be postive for Wolbachia. |
Water Boatman