Road Side Swallows

April 2, 2021

Sample information
Picture
Photos by: Mylene .M Tyson. E
Location
Collection date 03/23/2021
Captive / Cultivated? Wild-caught
Group Venture High School
Observations

I had went to antelope island and the weather was a lil windy and cold I had found my bug on a rock
Putative identification Arthropoda Myriapoda Chilopoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Larva
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

we followed the recommended conditions for wolbachia primers only we used the standard reagent from miniPCR

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

it matched all the positives but it looked a little off to me but it still looked really good
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chilopoda was found to be postive for Wolbachia.
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