Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/25/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Scientists in Residence |
| Observations | It was a sunny day. The bug was found dead on a sidewalk. Springtime was here, and one of the wings was gone. |
| Putative identification | Arthropoda Insecta Coleoptera Coccinellidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | I spilled some of the buffer when I was extracting the DNA. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | My bug was dead when it was found. We were not able to extract the DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
N/A
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid