Paper Wasp

April 12, 2025

Sample information
Picture
Photos by: Marium H
Location
Collection date 01/23/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Spring 2025 faculty
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes dominula

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Medium confidence level because I had a strong Wolbachia gel band, but did not any DNA, leading to no sequencing.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polistes dominula was found to be negative for Wolbachia.
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