MAC-CT Hymenoptera

April 15, 2025

Sample information
Picture
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location In front of University Building
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I made a prediction that my insect would not be infected with Wolbachia. After the gel electrophoresis testing, my results were correct. Also, according to a graph given to us, my order of insect rarely has Wolbachia present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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