MAC-RAP-Blattodea

April 15, 2025

Sample information
Picture
Photos by: Rebecca Proenza
Location
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Time of Collection: 2:50pm
Location of Collection: in a pine tree near the library, shaded area.
Description of Collection Site: inside tree bark, dry wood,  at the base of the tree.
Method of Collection: Forceps and gloved hands
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Along with my efforts in this project, my confidence level is increased because my results were very clear, accurate and precise. Also with the approval and good comments from my professor, I believe I achieved accurate results.
Wolbachia 16S sequence
Arthropod COI sequence
GgGTCTCTGATTGGAGACGACCAGATCTACAACGTcatCGTTACCGCCCA CGCATTTGTTATAATCTTCTTTATGGTtagtccaccaaTATCtttaggAT ttgttgaCcaaCTAGTACCACTTATATTAGGAGCCCCAGATATGGCATTC CCACGAATAAACAACATAAGATTTTGATTACTCCCACCATCATTAACTCT ACTACTCACTAGTAGAACTGTAGAAAGTGGAGCAGGGACGGGATGAACAG TTTATCCCCCTCTTGCAAGAGGTATTGCCCATGCAGGAGCATCTGTAGAC TTAGCCATCTTCTCCCTACACCTAGCAGGTGTATCATCAATCCTAGGGGC AGTAAACTTTATCTCAACAACAATCAACATAAAACCAAAAAACATAAAAC CCGAACGAATCCCACTATTTGTATGATCTGTCGCCATCACGGCCCTACTC CTACTATTGTCCCTGCCAGTGTTGGCCGGAGCAATCACCATACTACTAAC CGACCGCAACCTAAATACATCATTCTTTGATCCAGCCGGAGGTGGAGATC CAATTCTATACCAACACTTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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