MACNCO Hemiptera

April 15, 2025

Sample information
Picture
Photos by: Noah O
Location
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insect was caught on a tree on Georgia southern university campus.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident that all procedures were performed correctly.
Wolbachia 16S sequence
AGacCCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGA TTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGC TTTTAAGGGATTAGCTTAGCCTTGCGACTTTGCAGCCCATTGTAGCCACC ATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACAT CATCCCCACCTTCCTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCA GCATTACCTGATGGTAACTAAGGATGAGGGTTACGCTCGTTGCGGGACTT AACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGT GAAATCCGGCCGAACCGACCCTATCCCTTCGAATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TGATCAGGTATAGTTGGGTCATCCTTAAGATGAATTATTCGAATTGAACT GGGCCAGCCAGGTTCATTTATTGGAGATGATCAAATTTATAATGTAATTG TAACTGCTCATGCATTTATTATAATTTTCTTTATAGTTATGCCAATTATA ATTGGAGGTTTTGGTAATTGGCTAGTACCTTTAATAATTGGTGCACCAGA TATAGCATTCCCCCGAATAAATAATATAAGTTTTTGGTTATTACCCCCAT CATTAACCCTACTATTAAGAAGTAGCATGGTTGAAATAGGGGCAGGAACA GGTTGAACTGTTTATCCTCCATTATCAAGCAATATTTCACATAGAGGGGC TTCAGTAGATTTAGCTATTTTTTCCCTCCATTTAGCAGGAGTTTCATCTA TTTTAGGAGCAATTAATTTTATCTCAACTATTATAAATATACGCCCAGCA GGTATAATTCCTGAGCGAACTCCTCTCTTTGTATGATCAGTGGGAATTAC TGCTCTTCTATTACTTTTATCCTTACCTGTTTTAGCAGGGGCTATCACTA TATTATTAACTGATCGTAATTTTAATACTTCCTTTTTTGACCCCACTGGT GGAGGAGACCCTATCCTTTATCAACACTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be postive for Wolbachia.
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