Lasius niger NS1

May 7, 2025

Sample information
Picture
Photos by: Natalie S
Location
Collection date 03/05/2025
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found it in a little forest, about 3 pm, in a field path with gravel, at a sunny spot. It was about 10-15 degrees. I didn’t find alot of them, just one. The one I collected
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods
Extraction kit Instagene Matrix
DNA extraction location Body without legs
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected, there were no problems. I saw the CO-DNA band.

I am sad, because I wanted my ant to be infected, but it turned out it wasn’t :(
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence
Summary The Lasius niger was found to be negative for Wolbachia.
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