NV 74 Dobsonfly

April 11, 2021

Sample information
Picture
Location
Collection date 10/05/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations
  • lived in shady grass
  • possibly flying
  • midday
Putative identification Arthropoda Hexapoda Insecta Megaloptera Corydalidae

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Corydalidae was found to be negative for Wolbachia.
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