Ground beetle

Sample information

Photos by: Emma K
Collection date 03/23/2021
Captive / Cultivated? Wild-caught
Group Venture High School

from a distance it looked, small, shinny, and smooth, it was black and had a hard exoskeleton 

When I took a look up close he was a lot cooler, the legs were kind of curled, and his back had these grayish white spots that I did not spot before, there are these little hare like things on each of its legs he looks kind of like a spider but black, round, and has less body parts

Putative identification Arthropoda Hexapoda Insecta Coleoptera


Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

we followed recommended contentions for wolbachia primer’s we only used the standerd reagent from miniCPR


Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

the band was very faint

that it was hard to tell whether or not there was wolchaia but, it was only because I did not have enough DNA left

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be postive for Wolbachia.
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