NV ROLYPOLY 77

April 15, 2021

Sample information
Picture
Photos by: Neuqua
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

It was crawling amongst the crevices in a rotting wooden log. When disturbed it rolled up. It has 14 legs. It is a dark grey/blackish color. It has lines on its back to allow it to roll up. 
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band on the gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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