Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Longwood University |
| Observations | |
| Putative identification | Arthropoda Insecta Orthoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | DNA extraction: I made sure to crush the arthropod well, I dissected out the abdomen and excluded the exoskeleton. Minor issues: During the DNA extraction I had to spin down my sample multiple times due to the consistency of the extracted DNA, the washes were not working and we were eventually able to spin it down and wash it after multiple attempts. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I amplified the correct organism and it was not infected, there were minor issues during the PCR process, although the DNA and data was extracted and amplified. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Orthoptera was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica