Eastern Carpenter Bee

April 28, 2025

Sample information
Picture
Photos by: Karmin O.
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

The arthropod was caught near wildflowers Lake Mayes. It has large eyes and abdomen with large wings that extend past the abdomen. It was mostly black with a little yellow on the thorax.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Apidae Xylocopa

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

DNA extraction: The arthropod was cut at the abdomen and the contents were grounded with a pestle thoroughly.

Loading Gel Lanes:

lane 1- 100bp DNA ladder

lane 2-7- other group members’ samples

lane 8-9- my arthropod and Wolbachia samples

lane 10- DNA positive control

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All control bands worked, and the band for the arthropod gene PCR product was visible. I did not encounter any problems with the protocol. Therefore, I am confident that my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Xylocopa was found to be negative for Wolbachia.
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