Eastern Tailed-Blue Butterfly

April 28, 2025

Sample information
Picture
Photos by: Emma S.
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

Location: Wildflower field near Lake Mayes

Season: Fall

Temperature: 78 degrees F

Habitat: Terrestrial
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Lycaenidae

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected. I did not have any problems with the protocol, and I was not surprised by any bands on the gel. Therefore, I am confident my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Lycaenidae was found to be negative for Wolbachia.
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