Fragile Forktail

April 28, 2025

Sample information
Picture
Photos by: Emily B.
Location
Collection date 10/31/2025
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

The arthropod was collected on fall day with a temperature of 78º F. Found near a lake with flowers.
Putative identification Arthropoda Hexapoda Insecta Odonata Coenagrionidae Ischnura Ischnura posita

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

DNA extraction: I made sure to crush the abdomen well. I dissected the abdomen out and made sure to not include the exoskeleton.

Gel Electrophoresis lanes for CO1 arthropod PCR.

1.100bp Ladder

8. The tenth well was my arthropod sample.

9. The ninth well was my wolbachia sample.

10. The tenth well contained the positive and negative PCR controls.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked as expected. I had no controls with the protocols and was not surprised by any bands on the gel. Therefore, I am confident my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Ischnura posita was found to be negative for Wolbachia.
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