Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Longwood University |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis |
Methods |
|
| Extraction kit | promega wizard SV |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Ethidium Bromide |
| Gel images |
|
| Protocol notes | The KOCO1 well is positive for CO1 gene The KOW well is negative for Wolbachia The control DNA well is the positive control for CO1 gene and 16s rRNA gene The last well is the 1000 bp DNA ladder |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The organism was identified correctly. I had no trouble with the protocol and no results were unexpected. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Harmonia axyridis was found to be negative for Wolbachia. |
Baetis_C2.2
Formica_C11
Philaenus sp._C8
Ichneumonidae_A12
Formica_A11