Sample information
Picture	Photos by: Kendra D
Location
Collection date	10/31/2024
Captive / Cultivated?	Wild-caught
Group	Longwood University
Observations
Putative identification	Arthropoda Insecta Orthoptera Acrididae Melanoplus
Methods
Extraction kit	promega wizard SV
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	0.25 TAE
DNA stain	Ethidium Bromide
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	All my controls worked as expected and i was not surprised at any of the bands that appeared.
Wolbachia 16S sequence
Arthropod COI sequence	Download AB1
Summary	The Melanoplus was found to be negative for Wolbachia.