Goldenrod Solider Beetle

April 28, 2025

Sample information
Picture
Photos by: Emily S
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus

Methods
Extraction kit promega wizard SV
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 0.25 TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

A 1% agarose at 110V for 30 minutes, all well contained10 μL. 1) ESO arthropod (CO1) PCR reaction, 2)  ESO Wolbachia (W) PCR reaction, 3) Kendra CO1 PCR reaction, 4) Kendra W PCR reaction, 5) Saad CO1 PCR reaction, 6) Saad W PCR reaction, 7) Kassidy CO1 PCR  reaction 8) Kassidy W PCR reaction 9) Positive control from both groups 10) 100bp NEB Ladder. 

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My controls worked as expected. A band was amplified for the arthropod CO1 PCR but my arthropod was not identify  correctly. There was an error somewhere through the process but it’s unknown where the error happened. Overall, I am not confidence if my arthropod was infected with Wolbachia. 
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Chauliognathus pensylvanicus was found to be negative for Wolbachia.
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