Pure green sweat bee

April 28, 2025

Sample information
Picture
Photos by: Grace C.
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

It was caught at a wildflower field near a lake in the Fall.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Halictidae Augochlora Augochlora pura

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There was a clear band for Wolbachia on the gel electrophoresis for the sample as well as the control for a known Wolbachia positive organism. The genetic results matched the organism’s genome exactly to Augochlora pura.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Augochlora pura was found to be postive for Wolbachia.
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