Lasius niger NB2

May 14, 2025

Sample information
Picture
Photos by: Nando B.
Location
Collection date 03/19/2025
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found the Lasius niger nearby woodstacks in the forest directly by a path. The weather was sunny and it was around 15 degrees outside. The place was very dry and full of wood chips. It was in the afternoon, about 3pm. There were a lot of leaves around the place, where I found the Lasius niger. And it was autumn.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods
Extraction kit Instagene Matrix
DNA extraction location Body without legs
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There is always a possibility to make a mistake and I am not quite sure, if i didn’t puncture the gel on this line
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Lasius niger was found to be negative for Wolbachia.
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