NV 75 Beetle

April 15, 2021

Sample information
Picture
Location No map location data available.
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

The beetle was found in the mulch underneath the soil. It was 54 degrees outside.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel didn’t show a clear band for the Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Coleoptera was found to be negative for Wolbachia.
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