Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/04/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Neuqua Valley High School |
| Observations | Was sitting near sprouting point of grass, little movement, resting. Started hopping upon being disturbed. Was not exhibiting any abnormal behavior. |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus Melanoplus ponderosus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Very clear and dark bands in the W-spec gel image at the proper bp length for Wolbachia. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence |
|
| Summary | The Melanoplus ponderosus was found to be postive for Wolbachia. |
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