NV 62 Spur-Throated Grasshopper

April 15, 2021

Sample information
Picture
Photos by: Jack K
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

Was sitting near sprouting point of grass, little movement, resting. Started hopping upon being disturbed. Was not exhibiting any abnormal behavior.
Putative identification Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus Melanoplus ponderosus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Very clear and dark bands in the W-spec gel image at the proper bp length for Wolbachia.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence
Summary The Melanoplus ponderosus was found to be postive for Wolbachia.
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