Sample information |
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Picture |
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Location | |
Collection date | 10/04/2021 |
Captive / Cultivated? | Wild-caught |
Group | Neuqua Valley High School |
Observations | The insect I found was walking very quickly along the sidewalk but stopped when I covered it with the cup and even stayed still while I was up close to it, taking the picture. Also, the sidewalk it was roaming on was surrounded by a field of grass, so it most likely came from the grass. The insect was caught on a pretty cold day, right about the time the sun was setting. |
Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
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Extraction kit | Puregene (Qiagen) |
DNA extraction location | |
Single or Duplex PCR | |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | LB |
DNA stain | GelRed |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | I am fairly confident with my results because the insect band was definitely there, but it was smaller and fainter than others, meaning that there was not as much DNA being tested. |
Wolbachia 16S sequence | |
Arthropod COI sequence | Download AB1
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Summary | The Coleoptera was found to be negative for Wolbachia. |