NV 69 Strawberry Seed Beetle

Sample information

Picture
Location
Collection date 10/04/2021
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

The insect I found was walking very quickly along the sidewalk but stopped when I covered it with the cup and even stayed still while I was up close to it, taking the picture. Also, the sidewalk it was roaming on was surrounded by a field of grass, so it most likely came from the grass. The insect was caught on a pretty cold day, right about the time the sun was setting.

Putative identification Hexapoda Insecta Coleoptera

Methods

Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am fairly confident with my results because the insect band was definitely there, but it was smaller and fainter than others, meaning that there was not as much DNA being tested.

Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Coleoptera was found to be negative for Wolbachia.
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