Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/04/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Neuqua Valley High School |
| Observations | The insect I found was walking very quickly along the sidewalk but stopped when I covered it with the cup and even stayed still while I was up close to it, taking the picture. Also, the sidewalk it was roaming on was surrounded by a field of grass, so it most likely came from the grass. The insect was caught on a pretty cold day, right about the time the sun was setting. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Puregene (Qiagen) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | LB |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I am fairly confident with my results because the insect band was definitely there, but it was smaller and fainter than others, meaning that there was not as much DNA being tested. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman