|Captive / Cultivated?||Wild-caught|
|Group||Neuqua Valley High School|
The insect I found was walking very quickly along the sidewalk but stopped when I covered it with the cup and even stayed still while I was up close to it, taking the picture. Also, the sidewalk it was roaming on was surrounded by a field of grass, so it most likely came from the grass. The insect was caught on a pretty cold day, right about the time the sun was setting.
|Putative identification||Hexapoda Insecta Coleoptera|
|Extraction kit||Puregene (Qiagen)|
|DNA extraction location|
|Single or Duplex PCR|
|Gel electrophoresis system||Standard electrophoresis system|
|Explanation of confidence level||
I am fairly confident with my results because the insect band was definitely there, but it was smaller and fainter than others, meaning that there was not as much DNA being tested.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download AB1
|Summary||The Coleoptera was found to be negative for Wolbachia.|