Gryllinae GRP6 (2)

May 28, 2025

Sample information
Picture
Photos by: Isabella B.
Location
Collection date 05/01/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Found in a group under a basketball hoop in a backyard at night. It was roughly 65 degrees Fahrenheit.
Putative identification Arthropoda Insecta Orthoptera Gryllidae Gryllus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

Lane 1: Long Beach cricket 1, Lane 3: Long beach cricket 2, Lane 5: Hawthorne cricket 1, Lane 7: Hawthorne cricket 2

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

There were no bands on the gel for this cricket.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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