Coleoptera GRP12

May 27, 2025

Sample information
Picture
Photos by: Christian H.
Location
Collection date 05/13/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

caught deceased
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain Fast Blast
Gel images
Protocol notes

followed all labs

lanes (from left to right):

  1. beetle
  2. Roly- Poly
  3. PCR Standard
  4. Arthropod Positive Control
  5. Arthropod Negative Control
  6. DNA Positive Control
  7. empty
  8. empty

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

We are not fully confident in our results because there was some contamination found in our gel, and our PCR samples were not sent to the lab. We are at least a little confident that Wolbachia is found in this arthropod because bands appeared in the lane that contained our beetle sample. Our gel runs were pretty consistent as well, which gave us some confidence about the accuracy of our results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be postive for Wolbachia.
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