NV 52 Ant

April 14, 2021

Sample information
Picture
Photos by: Lucy L
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

ant was walking around on the cement with no other ants. there were some leaves and twigs around the location of the ant as well
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence
Summary The Formicidae was found to be negative for Wolbachia.
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