Sample information |
|
Picture |
|
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Location | |
Collection date | 10/04/2020 |
Captive / Cultivated? | Wild-caught |
Group | Neuqua Valley High School |
Observations | The grasshopper was just hopping around with the other grasshoppers there and vibing in the grass. |
Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
Extraction kit | Puregene (Qiagen) |
DNA extraction location | |
Single or Duplex PCR | |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | LB |
DNA stain | GelRed |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence | Download FASTA
Download AB1
|
Summary | The Orthoptera was found to be negative for Wolbachia. |