Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/04/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Neuqua Valley High School |
| Observations | I found my insect walking on the sidewalk. I covered it with the cup right away, and it immediately moved to the edge of the cup, as it was trying to get out. It was very active, and even when I put it in the bag, it was still moving around a lot. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Lygaeidae Nysius Nysius raphanus |
Methods |
|
| Extraction kit | Puregene (Qiagen) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | LB |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am very confident with the results because the controls worked and because the results were tested in a lab that has done this research several times with researchers that knows what they are doing. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Nysius raphanus was found to be negative for Wolbachia. |
Tenebrio molitor
Wasp-Hymenoptera sp
Pollenia pediculata,
Phyciodes tharos