NV 63 False Chinch Bug

April 14, 2021

Sample information
Picture
Photos by: Erin K
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

I found my insect walking on the sidewalk. I covered it with the cup right away, and it immediately moved to the edge of the cup, as it was trying to get out. It was very active, and even when I put it in the bag, it was still moving around a lot.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Lygaeidae Nysius Nysius raphanus

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident with the results because the controls worked and because the results were tested in a lab that has done this research several times with researchers that knows what they are doing.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Nysius raphanus was found to be negative for Wolbachia.
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