European Harvestmen

September 11, 2025

Sample information
Picture
Photos by: Dalani P.
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Long skinny legs, brown, circular but small abdomen with pattern on the back
Putative identification Arthropoda Arachnida Opiliones Phalangiidae Phalangium Phalangium opilio

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  1. DNA standard
  2. Common Hentz jumping Spider
  3. Praying mantis
  4. Wolbachia positive Wol + COI1
  5. Wolbachia Negative Wol + COI1
  6. gDNA control Wol + COI1
  7. Water control Wol + COI1
  8. EMPTY
  9. DNA standard
  10. European Harvestman Spider
  11. Banded Tussock Moth Caterpillar
  12. Wolbachia positive Wol + COI1
  13. Wolbachia Negative Wol + COI1
  14. gDNA control Wol + COI1
  15. Water control Wol + COI1
  16. EMPTY

 

DNA contamination between groups, skewing the results of the Harvestman spider identification, presenting it as a Northern Flatid Planthopper.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The Jumping Spider had two bands and tested positive for Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
CATTAACAGGTATAGTAGGAACTTCTTTAAGATTATTAATTCGAGCTGAATTAGGAAACCTGG TTTAATTGGAGATGATCAAATTTATAATACTATTTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAA TTATAATTGGAGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAAT ATAAGTTTTTGACTTTTACCCCCATCATTAACTTTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCCGGAACAGGATG AACAGTTTATCCACCCCTTTCATCTAATATTGCCCATGGAGGTAGATCAGTAGATTTAGCTATTTTTTCCCTTCATTTGG CAGGTATTTCTTCAATTTTAGGAGCAATTAATTTTATTACTACAATTATTAATATACGATTAAATAACTTATCTTTTGAT CAAATACCTTTATTTGTTTGAGCTGTAGGAATTACAGCATTTCTATTATTACTTTCATTACCTGTATTAGCAGGAGCTAT TACAATATTACTAACTGATCGAAATTTAAATACATCATTTTTCGACCCTGCTGGAGGAGGAGATCCTATTCTTTACCAAC ATTTATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Phalangium opilio was found to be negative for Wolbachia.
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