Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | It’s gray and small, rolled up, has little legs. |
| Putative identification | Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Lane 2: MQAM03A+W1 Lane 3:MQAM03A+W2 Lane 4:MQAM03Pos Lane 5:MQAM03Neg Lane 6:MQAM03DNA Lane 7: MQAM03water |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Our arthropods did not have any bands, which indicates an issue when we extracted the DNA, which makes me not fully confident in the results of whether or not Wolbachia was present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidium vulgare was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica